产品名称

β-Amylase from barley

别 称

1,4-α-D-Glucan maltohydrolase

含 量

Type II-B, Crude, 20-80units/mg protein (biuret); protein: ≥60% by biuret

CAS  NO.

9000-91-3

MDL  number

MFCD00081391

适用范围

生物试剂

l  生物学信息

生物活性/药理作用

β-Amylase hydrolyzes the α-(1,4) glucan ages in polysaccharides of three or more α-(1,4) ed D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. Pure, crystalline β-amylase preparation consists of four isoenzymes with different isoelectric points. The enzyme polymerizes very rapidly through the sulfhydryl groups in the absence of reducing agents. p-Chloromercuribenzoate inhibits the polymerization and the enzymatic activity. The reducing agents mercaptoethanol or dithiothreitol can completely restore the activity.

应 用

β -Amylase is used to hydrolyze α bonds of α-ed polysaccharides, such as starch and glycogen. β -Amylase, has been used in various plant studies, such as carbon starvation studies in Populus tremuloides. β -Amylase, from barley, has been used to study how pressure and temperature affect catalytic activity .

有效单位定义

One unit will liberate 1.0mg of maltose from starch in 3 min at pH4.8 at 20°C.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov