产品名称

α-Galactosidase I, Alkaline from Cucumis melo

一般描述

Alkaline α-Galactosidase I is a glycoside hydrolase, separated from the extracts of melon fruits. The activity of this enzyme is found to increase during the early stages of ovary development and fruit set.

别 称

α-D-Galactoside Galactohydrolase, Melibiase

含 量

≥85%(SDS-PAGE); ≥20units/mg protein;  expressed in E. coli

CAS  NO.

9025-35-8

分子量

apparent mol wt ~84 kDa by SDS-PAGE

MDL  number

MFCD00130486

适用范围

生物试剂

l  理化信息

外 观

冻干粉

物理形态

The product is supplied as a lyophilized powder containing Tris-HCl buffer salts, DTT, EDTA, and NaCl.

l  生物学信息

生物活性/药理作用

α-Galactosidase form I from melon may play a significant role in photoassimilate partitioning in sink tissue. The form I enzyme has a preferred activity with raffinose and significant activity with stachyose. A unique feature of this enzyme is that it exhibits weak product inhibition by galactose. This enzyme shows a preference for raffinose and also activity with stachyose. Unlike other a-Galctosidases, this enyzme also shows weak inhibition by galactose.

应 用

Alkaline α-Galactosidase I was used to assay enzyme activity with 2 mmp-nitrophenyl-α-d-galactoside as substrate at pH6.5 to compare with the enzyme activity of α-Gal A isolated and purified from Sf-9 insect cells infected with a recombinant baculovirus encoding normal α-Gal A gene.

样品准备说明

Reconstitution with 0.9ml of 0.05% (v/v) TWEENÒ 20 aqueous solution will yield a solution containing ~0.5mg-protein/ml, 50mM Tris-HCl, pH7.5, 1mM DTT, 0.1mM EDTA, 200mM NaCl, and 0.05% TWEEN 20.

有效单位定义

One unit will hydrolyze 1.0μmole of p-nitrophenyl α-D-galactoside to p-nitrophenol and D-galactose per minute at  pH7.8 at 30 °C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.  https://www.ncbi.nlm.nih.gov