产品名称

β-Galactosidase from Escherichia coli

一般描述

β-Galactosidase is a tetramer consisting of four equal subunits of 135,000 Da each. It is a sulfhydryl containing enzyme, with 19 cysteine residues per subunit.

别 称

β-D-Galactoside galactohydrolase, Lactase

含 量

Grade VI, 250-600units/mg protein; Protein, ≥50% biuret   

CAS  NO.

9031-11-2

MDL  number

MFCD00130623

适用范围

生物试剂

l  理化信息

外 观

冻干粉

物理形态

Partially purified; contains Tris buffer salts, magnesium chloride, DL-dithiothreitol and 2-mercaptoethanol

l  生物学信息

生物活性/药理作用

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

应 用

β-Galactosidase is conjugated to an antibody that specifically recognizes a target molecule (enzyme immunoassay or EIA). β-Galactosidase is also used as a reporter enzyme to monitor the level of gene of a promoter.

有效单位定义

One unit will hydrolyze 1.0μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH7.3 at 37°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov