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β-Glucuronidase from Helix pomatia

商品编号: S0524
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  • S0524-1ML

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    816

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    1495

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    2847

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国外库存,货期2-4周,具体详情请联系销售人员。

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详细介绍

l  基本信息

产品名称

β-Glucuronidase from Helix pomatia

一般描述

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

别 称

β-D-Glucuronide glucuronosohydrolase

含 量

Type HP-2, ≥100,000units/mL

CAS  NO.

9001-45-0

MDL  number

MFCD00130629

适用范围

生物试剂

l  理化信息

外 观

水溶液

物理形态

Aqueous solution in ~1.0M ammonium sulfate with 3mM sodium azide as preservative.

l  生物学信息

生物活性/药理作用

Used for the hydrolysis of glucuronide conjugates in urinary bolite analysis

β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

β-Glucuronidase from Helix pomatia is a mixture of enzymes derived from the Roman snail.

Optimal pH:

• Glucuronidase activity: 4.5 to 5.0

• Sulfatase activity: ~6.2

Inhibitors: D-glucuronic acid, D-galacturonic acid, D-glucaro-1, 4-lactone

应 用

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene .

β-glucuronidase was used in enzymic  hydrolysis of tissue homogenates for liquid chromatography-electrospray ion trap mass spectrometry (LC/MSn) analysis, to study the structures of degradation products of baicalin.

有效单位定义

One modified Fishman unit will liberate 1.0μg of phenolphthalein from phenolphthalein glucuronide per hr at 37°C at pH5.0 (30 min assay).

Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0μmole p-nitrocatechol sulfate per hr at pH5.0 at 37°C.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究,不得作为药物使用,不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov