产品名称

β-Glucuronidase from Helix pomatia

别 称

β-D-Glucuronide glucuronosohydrolase

含 量

Type H-3, ≥90,000units/mL; ≤1,000units/mL sulfatase

CAS  NO.

9001-45-0

MDL  number

MFCD00130629

适用范围

生物试剂

l  理化信息

外 观

水溶液

物理形态

Aqueous solution in ~1.0M ammonium sulfate with 3mM sodium azide as preservative.

l  生物学信息

生物活性/药理作用

Used for the hydrolysis of glucuronide conjugates in urinary bolite analysis

β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

β-Glucuronidase from Helix pomatia is a mixture of enzymes derived from the Roman snail.

Optimal pH:

• Glucuronidase activity: 4.5 to 5.0

• Sulfatase activity: ~6.2

Inhibitors: D-glucuronic acid, D-galacturonic acid,D-glucaro-1,4-lactone

应 用

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene .

有效单位定义

One modified Fishman unit will liberate 1.0μg of phenolphthalein from phenolphthalein glucuronide per hr at 37°C at pH5.0 (30 min assay).

Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0μmole p-nitrocatechol sulfate per hr at pH5.0 at 37°C.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov