产品名称

Hyaluronidase from bovine testes

别 称

Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

含 量

Type IV-S, mouse embryo tested, 750-3000units/mg solid

CAS  NO.

37326-33-3

MDL  number

MFCD00131351

适用范围

生物试剂

l  理化信息

外 观

白色至浅米色粉末

溶解性(25°C)

10 mg/mL in Water; Clear

l  生物学信息

生物活性/药理作用

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.

These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

应 用

Hyaluronidase is an enzyme derived from bovine testes. It is used to remove the cumulus mass from newly ovulated eggs, when preparing the eggs for in vitro culture.

Reconstitute 1vial of hyaluronidase with 3ml of M2 Medium or deionized water to obtain a 10mg/ml stock solution. Dilute stock solution with M2 Medium to obtain a final working concentration of approximately 300μg/ml.

Gonadotropins are used to superovulate 20 female mice. The mice are then mated and preimplantation stage embryos are retrieved and counted. The embryos are treated with hyaluronidase until the cumulus mass is dissociated from the embryos. The embryos are cultured, viewed daily and progress of the embryos is recorded.

有效单位定义

One unit will cause a change in % transmittance at 600nm of 0.330 per minute at pH5.35 at 37°C in a 2.0mL reaction mixture (45 minute assay).

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov