产品名称

β-N-Acetylglucosaminidase from bovine kidney

别 称

β-N-Acetyl-D-hexosaminide N-acetylhexosaminohydrolase, β-N-Acetylhexosaminidase

含 量

10-50units/mg protein

CAS  NO.

9012-33-3

MDL  number

MFCD00081402

适用范围

生物试剂

l  理化信息

外 观

硫酸铵悬浮液

物理形态

Suspension in 3.2M (NH⁴)²SO⁴, pH approx. 6.0

l  生物学信息

生物活性/药理作用

This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-ed N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates.

The activity of β-N-actylglucosaminidase may be determined with the chromogenic substrate p-nitrophenyl-N-acetyl-β-D-glucosaminide. β-N-actylglucosaminidase hydrolyzes the terminal nonreducing N-acetyl-D-hexosamine residues. This enzyme contains two predominant isozymes, Hex A, a heterodimer, and Hex B, a homodimer. N-acetylglucosamine, acetamide, N-2-acetamido-2-deoyglucosylamine, N-acetylnojirimycin, and N-acetyldeoxynojirmycin are known inhibitors.

应 用

Bovine kidney β-N-acetylglucosaminidase is a lysosomal enzyme used to hydrolyze N-acetyl-β-D-glucosaminides and N-acetyl-β-Dgalactosaminides. It is used in chemoenzymatic synthesis of oligosaccharides d on their effective transglycosylation of β-GlcNAc and  β-GalNAcc. It may be a useful tool to study Alzheimer’s Disease.

样品准备说明

This product is soluble in water (1mg/ml)

有效单位定义

One unit will hydrolyze 1.0μmole of p-nitrophenyl N-acetyl-β-D-glucosaminide to p-nitrophenol and N-acetyl-D-glucosamine per min at the pH4.25 at 25°C.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov