产品名称

O-Glycosidase from Streptococcus pneumoniae

别 称

Endo-α-N-acetylgalactosaminidase,O-Glycanase

含 量

recombinant, expressed in E. coli, ≥1.25U/ml

CAS  NO.

9032-92-2

MDL  number

MFCD00131003

适用范围

生物试剂

l  理化信息

外 观

缓冲水溶液

物理形态

Solution in 50mM sodium phosphate, pH7.5

l  生物学信息

生物活性/药理作用

Releases unsubstituted Ser- and Thr-ed  β-Gal-(1→3)-α-GalNAc (Core 1 type O-glycan) from glycoproteins. Substitutions of the disaccharide core with sialic acid, lactosamine (galactose-N-acetyl glucosamine), or fucose will block hydrolysis and prevent the liberation of the oligosaccharide from the protein. Pretreament with glycolytic enzymes to remove substituent saccharides from the O-glycan may be needed prior to cleavage using O-glycosidase..

有效单位定义

One unit will hydrolyze 1μmole of p-nitrophenyl galacto-N-bioside (β-Gal-(1→3)-α-GalNAc-1→ΟC⁶H⁴NO²) per min at 37°C at pH6.5.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov