产品名称

Fpg Protein from Escherichia coli

一般描述

Formamidopyrimidine-DNA glycosylase (Fpg) is a DNA repair enzyme found in Escherichia coli, which contains one zinc atom. Proximal to its C-terminal, it contains a zinc-finger motif of CC/CC  type.

别 称

Fapy DNA glycosylase, Formamidopyrimidine DNA glycosylase, MutM

含 量

≥90% (SDS-PAGE), recombinant, expressed in E. coli, >20,000units/mg protein

CAS  NO.

78783-53-6

分子量

mol wt 30.2 kDa (269 amino acids, predicted from the nucleotide sequence)

MDL  number

MFCD02264758

适用范围

生物试剂

l  理化信息

外 观

无色甘油水的缓冲溶液

物理形态

Solution in 50% glycerol containing 50mM potassium HEPES, pH7.5, 1mM DTT, 1mM EDTA, and 200mM NaCl.

l  生物学信息

生物活性/药理作用

Formamidopyrimidine-DNA glycosylase (Fpg) cleaves double-stranded DNA containing the damaged 8-oxo-7,8-dihydroguanine. It functions as an N-glycosylase and apurinic/apyrimidinic lyase.

应 用

Fpg Protein from Escherichia coli has been used for the assessment of DNA oxidative damage using comet assay.

有效单位定义

One unit will cleave 50% of 0.5 pmol of double-stranded DNA oligomer substrate (8-oxoguanine−mutated) in 10min at 25°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.  https://www.ncbi.nlm.nih.gov