产品名称

Aminopeptidase from Aeromonas proteolytica

一般描述

A zinc-containing enzyme.

含 量

50-150units/mg protein; Protein, ~40%biuret  

CAS  NO.

37288-67-8

MDL  number

MFCD00166325

适用范围

生物试剂

l  理化信息

外 观

白色至白色/黄色冻干粉末

物理形态

Lyophilized powder containing tricine buffer, pH8.0, zinc chloride and stabilizer.

溶解性(25°C)

H²O: soluble 0.9-1.1mg/mL,clear, colorless

l  生物学信息

生物活性/药理作用

Aminopeptidase from Aeromonas proteolytica is involved in protein maturation, hormone production and peptide digestion.

Aminopeptidase from Aeromonas proteolytica is a lloenzyme, which contains 2 atoms of Zn₂₊ in a single polypeptide with an approximate molecular weight of 29.5 kDa as determined by sedimentation. This enzyme has a high degree of stability, being stable even at temperatures of 70°C for several hours. Partial inactivation occurs in 8M urea. Maximum stability and activity are between pH 8.0-8.5. Aminopeptidase  from Aeromonas proteolytica can function as an esterase.

应 用

Aminopeptidases are a family of widely distributed proteases, which may be used to study many significant biological processes such as protein maturation, hormone production, and peptide digestion. The enzyme has been used to measure the kinetic rate constant for the binding of bestatin, a general protease inhibitor, to aminopeptidase.

样品准备说明

Dissolves in water at 0.9-1.1mg/mL concentration to form a clear, colorless solution.

有效单位定义

One unit will hydrolyze 1.0μmole of L-leucine p-nitroanilide to L-leucine and p-nitroaniline per min at pH8.0 at 25°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov