产品名称

Aminopeptidase I from Streptomyces griseus

别 称

Leucine Aminopeptidase IV

含 量

≥200units/mg protein; Protein, 40-60%Lowry

CAS  NO.

9031-94-1

MDL  number

MFCD00166325

适用范围

生物试剂

l  理化信息

外 观

白色至淡黄色冻干粉末

物理形态

Contains calcium acetate

l  生物学信息

生物活性/药理作用

Aminopeptidase I from S. griseus has a fairly broad specificity, being able to remove the N-terminal residue of most proteins, except where the penultimate residue is an imino acid. It contains two Zn₂₊ binding sites. Aminopeptidase I from S. griseus is inhibited by 1,10-phenanthroline and is activated six-fold by Ca₂₊, which also stabilizes it against heat inactivation. This monomeric zinc lloprotein has an isoelectric point (pI) of 5.4.

应 用

Aminopeptidase I from Streptomyces griseus may be used as a reagent for the analysis of protein structure and as a model for studies of proteolytic enzyme activation by calcium ions. It may be used as a reagent in the assay of endoprotease activities with a synthetic substrate in a two-stage assay. The lyophilized powder also contains calcium acetate.

样品准备说明

Reconstitute in 20mM tricine, pH8.0, with 0.05% bovine serum albumin. Dilute the enzyme with the reconstitution buffer to 0.15-0.3 U/mL for a working concentration. Solutions should be prepared fresh prior to use.

有效单位定义

One unit will hydrolyze 1.0μmole of L-leucine-p-nitroanilide to L-leucine and p-nitroaniline per min at pH8.0, 25°C and 3.0mM substrate concentration.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov