产品名称

Prolidase from porcine kidney

别 称

Aminoacyl-L-proline hydrolase, Imido Dipeptidase, Prolidase, Proline dipeptidase

含 量

≥100units/mg protein; Protein, 20-60%Lowry

CAS  NO.

9025-32-5

MDL  number

MFCD00132079

适用范围

生物试剂

l  理化信息

外 观

冻干粉

物理形态

Supplied as a lyophilized powder containing Tris buffer salt and MnCl².

l  生物学信息

生物活性/药理作用

Prolidase is an enzyme that catalyzes the hydrolysis of the imide bond between an α-carboxyl group and proline or hydroxyproline. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues.

应 用

Prolidase is has an important role recycling of proline and collagen production. It is used to study mutations in the PEPD gene that cause prolidase deficiency. It is used to hydrolyze proteins with C-terminal proline or hydroxyproline residues. Prolidase, product P6675 from porcine kidney, has been used to hydrolyze peptide bonds from the amino terminus when studying enzymatic methylation of membrane proteins.

有效单位定义

One unit will hydrolyze 1.0μmole of Gly-Pro per min at pH8.0 at 40°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov