产品名称

Cathepsin C from bovine spleen

别 称

Dipeptidyl aminopeptidase, Dipeptidyl peptidase I

含 量

Type X, ≥5units/mg protein; Protein, ≥25% biuret

CAS  NO.

9032-68-2

MDL  number

MFCD00081490

适用范围

生物试剂

l  理化信息

外 观

冻干粉

物理形态

Lyophilized from a 1M sodium chloride solution.

l  生物学信息

生物活性/药理作用

Cathespin C is a dipeptidyl aminopeptidase that can sequentially remove dipeptides from a peptide chain with an unsubstituted N-terminus. The enzyme exhibits a preference for glycine and proline as N-terminal aminoacids. Substrates that have an N-terminal lysyl or arginyl residue, or a penultimate proryl residue are not targeted by this enzyme.The endopeptidase activity requires the presence of halide ions and sulfydryl activators.

应 用

Cathepsin C has been used in a study that demonstrated the potential of a proteomics approach to identify novel proteins expressed by extravillous trophoblast and to uncover the mechanisms leading to disease states in pregnancy. Cathepsin C has also been used in a study to evaluate biodegradable thermogels.

The enzyme from Sigma has been used in the activation of granzyme k (Gzmk) precursor from E. coli. Granzymes are granule-stored lymphocyte serine proteases that are implicated in T- and natural killer cell-mediated cytotoxic defense reactions.

注 意

Unstable. Keep frozen.

有效单位定义

One unit will produce μmole of Gly-Phe-NHOH from Gly-Phe-NH² and hydroxylamine per min at pH6.8  at 37°C using DL-phenylalanine hydroxamic acid as the standard. In addition to its hydrolytic properties, cathepsin C catalyzes the polymerization of dipeptide amides.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov