产品名称

Dipeptidyl Peptidase IV human

别 称

CD26, DPPIV, Dipeptidyl aminopeptidase IV, Glycoprotein GP110

含 量

recombinant, expressed in Sf9 cells; ≥4,500units/μg protein

MDL  number

MFCD00166456

适用范围

生物试剂

l  理化信息

物理形态

Supplied as a solution in 45mM Tris-HCl,  pH8.0, 124mM NaCl, 2.4mM KCl, 225mM imidazole, 10% glycerol and 3mM DTT.

l  生物学信息

生物活性/药理作用

Native DPPIV is a ubiquitous type II transmembrane glycoprotein and a serine protease of the S9 prolyl-oligopeptidase family. In vivo, it is synthesized with a signal peptide, which functions as the membrane anchoring domain. There is an 88% sequence homology between the human and porcine kidney enzymes. Both exist as homodimers with a subunit molecular weight of ~30 kDa. The high mannose 100 kDa DPPIV precursor is processed in the Golgi to yield a124 kDa heavily N-and O-ed mature glycoprotein. It is then sorted to the apical membrane through the concerted action of both N- and O-ed glycans and its association with lipid microdomains. The porcine enzyme contains 18.3% carbohydrates, which the glycan composition is 0.9% fucose, 3.4% mannose, 5.1% galactose, 8.2% glucosamine, and 0.7% sialic acid. DPPIV is highly expressed on endothelial cells, epithelial cells, and lymphocytes. It is also present in plasma in its soluble form.

应 用

Human dipeptidyl peptidase IV has been used in a study to assess the interactive hemodynamic effects of its inhibition as well as the angiotensin-converting enzyme inhibition. Human dipeptidyl peptidase IV has also been used in a study to identify and characterize the 100 kDa High Molecular Weight Hymenoptera Venom Allergens Api m 5 and Ves v 3.

有效单位定义

One unit will hydrolyze 1.0 picomole of Ala-Pro-AMC per minute at pH7.5 at 25°C

l  存储

存储温度

−70°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov