产品名称

Carboxypeptidase A from bovine pancreas

别 称

Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase

含 量

(Type II-PMSF treated), ≥50units/mg  protein

CAS  NO.

11075-17-5

分子量

mol wt ~35 kDa

MDL  number

MFCD00130718

适用范围

生物试剂

l  理化信息

外 观

白色至类白色悬浮水溶液

l  生物学信息

生物活性/药理作用

Carboxypeptidase as isolated from bovine pancreas glands is a lloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by beta-phenylpropionate and indole acetate.

应 用

Carboxypeptidase A from bovine pancreas has been used in a study to investigate the of a soluble and activatable form of bovine procarboxypeptidase A in Escherichia coli. Carboxypeptidase A from bovine pancreas has also been used in a study to investigate the isolation and partial characterization of precursor forms of ostrich carboxypeptidase.

The enzyme from Sigma has been used as a comparison to study the specificity of rhizium anisopliae carboxypeptidase A (MeCPA). MeCPA had been genetically engineered to facilitate the removal of polyhistidine tags from the C-termini of recombinant proteins. It has also been used to de-tyrosinate α-tubulin, in vitro, in order to induce high affinity to ethyl-N-phenylcarbamate (EPC)  sepharose.

样品准备说明

Treated with phenylmethylsulfonyl fluoride to eliminate trypsin and chymotrypsin activity. Dialyzed and recrystallized: aqueous suspension with toluene added.

有效单位定义

One unit will hydrolyze 1.0μmole of hippuryl-L-phenylalanine per min at pH7.5 at 25°C.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov