产品名称

Carboxypeptidase G from Pseudomonas sp.

别 称

γ-Glutamyl hydrolase

含 量

≥3units/mg protein; Protein,~70% biuret

CAS  NO.

9074-87-7

MDL  number

MFCD00130719

适用范围

生物试剂

l  理化信息

外 观

白色至粉红色，黄色，橙色或棕色冻干粉末

物理形态

Contains sodium acetate salt.

溶解性(25°C)

H²O: soluble 0.5mg/mL

l  生物学信息

生物活性/药理作用

Carboxypeptidase G is a lysosomal, thiol-dependent protease, which progressively cleaves γ-glutamyl pteroyl poly-γ-glutamate yielding pteroyl-α-glutamate (folic acid) and free glutamate. It is considered highly specific for the γ-glutamyl bond, but not for the C-terminal amino acid of the leaving group. Molecular mass of this homodimer is approximately 90 kDa. The enzyme is activated by Zn₂₊ ions.

应 用

Carboxypeptidase G from Pseudomonas sp , or γ-Glutamyl hydrolase, has been used in a study to assess the role of the putidaredoxin COOH-terminus in P-450cam (cytochrome m) hydroxylations. Carboxypeptidase G from Pseudomonas sp. has also been used in a study to investigate the effects of nitric oxide on pemetrexed cytotoxicity via NO-cGMP signaling in lung adenocarcinoma cells.

样品准备说明

Chromatographically purified; Solutions should be prepared fresh prior to use.

有效单位定义

One unit will hydrolyze 1.0μmole of L-glutamic acid from (+)amethopterin per min at pH7.3 at 30°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov