产品名称

Carboxypeptidase B from porcine pancreas

别 称

Peptidyl-L-lysine(L-arginine) hydrolase, Protaminase

含 量

≥125units/mg protein; Protein, 40-70%

CAS  NO.

9025-24-5

MDL  number

MFCD00081476

适用范围

生物试剂

l  理化信息

外 观

冻干粉

物理形态

Contains HEPES buffer salts, zinc chloride and carbohydrate

l  生物学信息

生物活性/药理作用

Carboxypeptidase B is a proteolytic enzyme capable of rapidly hydrolyzing peptide bonds to release certain carboxyl-terminal basic amino acids from peptides and proteins. Its molecular mass is 34,300±600 Da. It contains one non-dialyzable gram atom of zinc per mole. The enzyme activity is inhibited by l chelating agents 1, 10-phenanthroline, 8-hydroxyquinoline-5-sulfonic acid, and 2,2’-dipyridyl.

应 用

Carboxypeptidase B has been used in a study to develop a non-invasive pregnancy assay for use in both captive and wild polar bears. Carboxypeptidase B has been used in a study that identified new potential biomarkers of acute pancreatitis.

Carboxypeptidase B has been used to develop homogeneous time-resolved fluorescence (HTRF) assay for measuring carboxypeptidase B activity in a miniaturized high-throughput screening format.It has been used to evaluate the impact of the C-terminal lysine(s) in human plasminogen binding to Bifidobacterium. The effect of treatment with carboxypeptidase B, which is a C-terminal lysine-specific endopeptidase, is measured using flow cytometry analysis.

样品准备说明

Treated with protease inhibitor, AEBSF, to eliminate serine protease activity.

有效单位定义

One unit will hydrolyze 1.0μmole of hippuryl-L-arginine per min at pH7.65 at 25°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov