产品名称

Pyroglutamate Aminopeptidase from Pyrococcus furiosus

别 称

L-Pyrrolidone carboxyl peptidase

含 量

recombinant, expressed in E. coli, ~90%(SDS-PAGE), ≥5.0units/mg protein

分子量

mol wt 24.072 kDa by amino acid sequence; mol wt 28 kDa by SDS-PAGE

MDL  number

MFCD00132139

适用范围

生物试剂

l  理化信息

外 观

冻干粉

物理形态

Lyophilized powder containing sodium phosphate

l  生物学信息

生物活性/药理作用

This enzyme is specific for N-terminal pyroglutamic acids. It cleaves the N-terminal pyroglutamic acid from proteins and peptides prior to Edman degradation. The optimal temperature range is 95 to 100°C and the optimal pH range is 6.0 to 9.0.

应 用

Thermostable aminopeptidase that liberates N-terminal pyroglutamic acid from proteins and peptides prior to Edman degradation.

Pyroglutamate Aminopeptidase, from Pyrococcus furiosus is a recombinant, thermostable aminopeptidase that is expressed in Escherichia coli. It is used to cleave pyroglutamic acid which allows analysis of N-terminal sequences of peptides.

Thermostable aminopeptidase has been used for the removal of pyroglutamate (pGlu) N-terminal blocking group, under reduced conditions, prior to N-terminal sequencing of purified cassiicolin.

样品准备说明

Reconstitute the vial of enzyme with 50μl of 50mM sodium phosphate, pH7.0, with 10mM DTT and 1mM EDTA. The  reconstituted solution should be stored at -20°C.

有效单位定义

One unit will hydrolyze 1μmol of pyroglutamate p-nitroanilide per minute at pH7.0 at 37°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov