产品名称

α-Chymotrypsin from bovine pancreas

别 称

TLCK-Chymotrypsin

含 量

TLCK treated to inactivate residual tryspin activity, Type VII, essentially salt-free, ≥40units/mg protein

CAS  NO.

9004-07-3

MDL  number

MFCD00130481

分子量

mol wt 25 kDa

适用范围

生物试剂

l  理化信息

外 观

冻干粉

溶解性(25°C)

1mM HCl: soluble 10mg/mL, clear

l  生物学信息

生物活性/药理作用

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) ed by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. Its pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca₂₊ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α₁-antitrypsin, α₂-macroglobulin, 10 mM Cu₂₊ and Hg₂₊.

应 用

α-Chymotrypsin has been used to determine the crystal structures of two homologous inhibitors (pars intercerebralis major peptide-C and pars intercerebralis major peptide-D2v) from the insect Locusta migratoria by forming a complex with the enzyme.

样品准备说明

TLCK treatment inactivates trypsin which may be present in chymotrypsin, without affecting the chymotrypsin activity.

有效单位定义

One unit will hydrolyze 1.0μmole of BTEE per min at pH7.8 at 25°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov