产品名称

α-Chymotrypsin from bovine pancreas

含 量

Type II,≥40units/mg protein; protein, ≥85%

CAS  NO.

9004-07-3

分子量

mol wt 25 kDa

MDL  number

MFCD00130481

适用范围

生物试剂

l  理化信息

外 观

白色至类白色冻干粉末

l  生物学信息

生物活性/药理作用

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

α-Chymotrypsin is a serine peptidase and  has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) ed by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is  8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca₂₊ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF),  N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α¹-antitrypsin, and α²-macroglobulin, 10mM Cu₂₊ and Hg₂₊.

应 用

α-Chymotrypsin has been used to study the structure-function relationship in glycosylated α-chymotrypsin using immobilized l-ion affinity chromatography (IMAC) and immobilized l-ion affinity capillary electrophoresis (IMACE).

样品准备说明

Produced from 3× crystallized chymotrypsinogen

有效单位定义

One unit will hydrolyze 1.0μmole of BTEE per min at pH7.8 at 25°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov