产品名称

α-Chymotrypsin−Agarose from bovine pancreas

含 量

2,000-3,500units/g agarose (One ml gel will yield 65-120units)

分子量

mol wt 25 kDa

MDL  number

MFCD00130481

适用范围

生物试剂

l  理化信息

外 观

白色冻干粉

物理形态

Stabilized with lactose

l  生物学信息

生物活性/药理作用

Serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met) on the carboxyl end of the bond.

α-Chymotrypsin is a serine peptidase and has 241amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) ed by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca₂₊ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10mM Cu₂₊ and Hg₂₊.

应 用

α-Chymotrypsin agarose from bovine pancreas has been used to study the purification and characterization of glucoamylase. α-Chymotrypsin agarose from bovine pancreas has also been used in a study to investigate molecular modeling for the design of a biomimetic chimeric ligand.

有效单位定义

One unit will hydrolyze 1.0μmole of N-acetyl-L-tyrosine ethyl ester (ATEE) per min at pH8.0 at 30°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov