产品名称

Endoproteinase Glu-C from Staphylococcus aureus V8

别 称

V8 Protease

含 量

≥50µg protein

适用范围

生物试剂, suitable for protein sequencing

l  理化信息

外 观

白色冻干粉

l  生物学信息

生物活性/药理作用

Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine endoprotease, which hydrolyzes peptide bonds at the carboxyl side of glutamyl and aspartyl residues. The specificity of Glu-C is dependent upon the buffer and pH employed as well as the structure around the potential cleavage site. In ammonium acetate (pH4.0) or ammonium bicarbonate (pH7.8), the enzyme preferentially cleaves glutamyl bonds; whereas, in phosphate buffer (pH7.8) Glu-C will cleave at either site. Glu-C is reported to be active in the presence of 0.2% SDS (sodium dodecyl sulfate) and in 4.0M urea.

应 用

• Endoproteinase Glu-C from Staphylococcus aureus V8 has been used for obtaining proteolytic cleavage fragments of the  S-layer protein (from Bacillus stearothermophilus ATCC12980) to perform affinity studies.

• It has been used for the enzymatic cleavage of native VSTx-3 (voltage sensor toxin 3) peptide for its sequence determination.

• It has been used for limited proteolysis of recombinant purified PimA (phosphatidylinositol mannosyltransferase).

• It has been used for the digestion of glycosylated hemoglobin for isotope dilution liquid chromatography-tandem mass spectrometry analysis.

样品准备说明

Reconstitute the lyophilized product in water.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov