产品名称

Elastase from porcine pancreas

别 称

Elastase from hog pancreas, Pancreatopeptidase E

含 量

Type IV, Protein 50-90%, ≥4.0units/mg protein (biuret)

CAS  NO.

39445-21-1

MDL  number

MFCD00130998

适用范围

生物试剂

l  理化信息

外 观

冻干粉

物理形态

Contains sodium carbonate.

l  生物学信息

生物活性/药理作用

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.

Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9 kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin. It is a serine protease with broad specificity as it cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides and p-dinitrophenyl diethylphosphate and high salt concentrations. It is extensively used in tissue and cell dissociation procedures. Elastase is effective in the isolation of Type II lung cells.

应 用

Elastase has been used to examine the extent of proteolytic degradation of BSA that is treated with hydroxyl radical. It has also been used to purify elastase-inhibitory lipid derivative from a cyanobacterium, Microcystis Ku2.

Elastase from porcine pancreas has been used in a study to assess the moleculars for human leucocyte elastase inhibition. Elastase from porcine pancreas has also been used in a study to investigate the molecular cloning and of serum calcium-decreasing factor (caldecrin).

样品准备说明

A further purification of Type III by affinity chromatography to reduce trypsin activity.

有效单位定义

One unit will hydrolyze 1.0μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH8.0 at 25°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov