产品名称

Elastase from porcine pancreas

一般描述

Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.

别 称

Elastase from hog pancreas, Pancreatopeptidase E

含 量

suitable for cell culture; ≥4units/mg protein

CAS  NO.

39445-21-1

MDL  number

MFCD00130998

适用范围

生物试剂

l  理化信息

外 观

白色至黄色至米色冻干粉末

l  生物学信息

生物活性/药理作用

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.

Enzyme that degrades elastin, a matrix component of tissues involved in stretching processes. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester.

应 用

Elastase from porcine pancreas is used for the following applications:

• Cell culture techniques

• ELISA techniques

• Used in assay for elastase inhibitory activity

有效单位定义

One unit will hydrolyze 1.0μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH8.0 at 25°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.  https://www.ncbi.nlm.nih.gov