产品名称

Elastase from human leukocytes

别 称

Lysosomal elastase

含 量

≥50units/mg protein (Bradford)

CAS  NO.

9004-06-2

MDL  number

MFCD00130997

适用范围

生物试剂

l  理化信息

外 观

白色至类白色冻干粉末

物理形态

Lyophilized from 0.05M sodium acetate (pH5.5) and 0.6M NaCl

l  生物学信息

生物活性/药理作用

Unlike pancreatic elastase the leukocyte enzyme has a preferential cleavage for the carboxyl side of valine, but will also cleave to a lesser extent after alanine. Natural substrates include elastin, cartilage proteoglycans, collagen types I, II, II and IV, and fibronectin.

应 用

Elastase has been used to digest  fibronectin. The results were compared with fibronectin digestion by crude human leukocyte homogenateto examine the presence of fibronectin peptides in saliva of patients with Sjögren′s syndrome.It has also been used as a reference to determine the elastase activity in cell lysates. This study examined the effect of all-trans retinoic acid on procoagulant and fibrinolytic activities of cultured blast cells. These blast cells were from patients with acute promyelocytic leukemia.

Elastase from human leukocytes has been used in a study that determined that fragments of Nle3-angiotensin(1-7) accelerate healing in dermal models.

有效单位定义

One unit will release one nanomole of p-nitrophenol per sec from BOC-L-alanine p-nitrophenyl ester at pH 6.5 at 37°C.

l  存储

存储温度

-20 °C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov