产品名称

Chymase human

别 称

Mast cell protease I

含 量

recombinant, expressed in Pichia pastoris; ≥40units/mg protein; 125.00-400.00µg Protein/ml

CAS  NO.

97501-92-3

MDL  number

MFCD00132173

适用范围

生物试剂

l  理化信息

外 观

无色透明液体

物理形态

Supplied as a solution in 20mM Tris, 0.8M NaCl and 25% glycerol, pH7.6

l  生物学信息

生物活性/药理作用

The enzyme rapidly converts angiotensin I to angiotensin II. Optimum pH for the enzyme activity is between 7.5 and 9.0. Enzyme activity is inhibited by soybean trypsin inhibitor, phenylmethylsulfonyl fluoride and chymostatin.

应 用

Chymase has been implicated in generation of angiotensin II and cleavage of big endothelin. Studies indicate it may be  involved in vascular proliferation, myocardial infarction and dermatitis.

Human chymase has been used in a study to assess the effects of Panax notoginseng flower extract on the TGF-β/Smad signal transduction pathway in heart remodeling. Human chymase has also been used in a study to investigate the blood glucose level and survival in streptozotocin-treated human chymase transgenic mice.

有效单位定义

One unit hydrolyzes one micromole of  N-benzoyl-L-tyrosine ethyl ester (BTEE) per minute at pH7.8 and 25°C. The assay buffer used to determine the enzyme activity contains 27mM Tris-HCl, pH7.8, with 150mM NaCl and 0.43mM BTEE.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov