产品名称

Trypsin Acetylated from bovine pancreas

一般描述

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

别 称

Acetyltrypsin

含 量

Type V-S, Protein (Biuret): 80 - 100%; ≥8,500 BAEE units/mg protein (biuret)

MDL  number

MFCD00082094

适用范围

生物试剂

l  理化信息

外 观

白色至灰白色粉末

l  生物学信息

生物活性/药理作用

Lys-acetylated trypsin is less susceptible to autolysis than the native form.

应 用

Trypsin has been used in a study to assess the ontogeny of the endocrine pancreas in the fetal/newborn baboon. Trypsin digestion and hydrogen/deuterium exchange support the transition between inward- and outward-facing conformations during the catalytic cycle of the bacterial multidrug ATP-binding cassette transporter.

样品准备说明

Prepared by the method of Labouesse, J. and Gervais, M., Eur. J.Biochem., 2, 215 (1967).

有效单位定义

One BAEE unit will produce a ΔA²⁵³ of 0.001 per min at pH7.6 at 25°C using BAEE as substrate. Reaction volume = 3.2 mL (1cm light path).

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov