产品名称

Trypsin from bovine pancreas

一般描述

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

含 量

TPCK Treated,≥10,000 BAEE units/mg protein; protein, ≥95%

CAS  NO.

9002-07-7

分子量

mol wt 23.8 kDa

MDL  number

MFCD00082094

适用范围

生物试剂

l  理化信息

物理形态

基本上无盐的白色冻干粉末

溶解性(25°C)

hydrochloric acid: soluble 1 mM, clear

l  生物学信息

生物活性/药理作用

Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide ages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity. Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.

应 用

For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins  have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestions. Additional applications include assessing crystallization by membrane-d techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.

Trypsin can be used to release adherent cells from tissue culture plates for passaging. Trypsin has been used in a study to assess the effects of macromolecular crowding on the structural stability of human α-lactalbumin. Trypsin has also been used in a study to investigate BN-PAGE analysis of Trichoderma harzianum secretome.

样品准备说明

TPCK treated

有效单位定义

One BAEE unit will produce a ΔA²⁵³ of 0.001 per min at pH7.6 at 25°C using BAEE as substrate. Reaction volume =3.2 ml (1 cm light path). One BTEE unit = 320 ATEE units.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov