产品名称

Factor X Activated (Xa) from bovine plasma

别 称

Factor Xa

含 量

<13mg/ml (UV); ≥50units/mg protein; ≥100units/ml

CAS  NO.

9002-05-5

MDL  number

MFCD00163610

适用范围

生物试剂

l  理化信息

外 观

无色透明的甘油水溶液

l  生物学信息

生物活性/药理作用

Factor Xa catalyzes the hydrolysis of the Arg-Thr and then Arg-Ile bonds in prothrombin to yield active thrombin.

The fairly strict recognition sequence is Ile-Glu (or Asp)-Gly-Arg-↓-X.

It may sometimes cleave at other basic residues, depending on the conformation of the target protein. Factor Xa will not cleave if a proline residue follows the arginine of the recognition sequence.

pH Optimum: 7.6-8.0

Temperature Optimum: 37°C

应 用

Fusion proteins are commonly expressed with a factor Xa cleavable Ile-Glu (or Asp)-Gly-Arg-↓-X sequence. Typically 1mg of fusion protein can be incubated with 10μg of factor Xa for 2.5 hours at 37°C.

有效单位定义

One unit of activated Factor X will  liberate 1.0μmole of p-nitroanilide from N-benzoyl-L-isoleucyl-L-glutamyl-L-glycyl-L-arginine-p-nitroaniline per minute at pH8.3 at 37°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.  https://www.ncbi.nlm.nih.gov