产品名称

Protease from Bacillus licheniformis

一般描述

The enzyme is found to be stable at pH8-10, retaining activity of up to 90% for 24 hours. It shows maximum activity at temperatures between 55-60°C.

别 称

Proteinase from Bacillus licheniformis, Subtilisin A

含 量

Type VIII, 7-15 units/mg solid

CAS  NO.

9014-01-1

MDL  number

MFCD00132092

适用范围

生物试剂

l  理化信息

外 观

白色至浅黄色和淡淡米色至浅米色冻干粉末

溶解性(25°C)

10mM NaAc (pH7.5) and 5mM CaAc: soluble, clear; H²O: soluble, colorless

l  生物学信息

生物活性/药理作用

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Protease, Product P5380, is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is active in some organic solvents such as dry octane.

应 用

The product has been used with other enzymes for in situ proteolysis to produce crystals suitable for structure determination. It has also been used in the process of isolation of subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria that can be used for functional in vitro studies. This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions.

有效单位定义

One unit will hydrolyze casein to produce color equivalent to 1.0μmole (181μg) of tyrosine per min at pH7.5 at 37°C (color by Folin-Ciocalteu reagent).

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov