产品名称

Protease from Bacillus licheniformis

一般描述

The enzyme hydrolyzes gelatin optimally at 50°C over the range of pH 8-10. It is stable between pH6-10 at 50°C. Commercially, it is used in laundry detergent formulations and also for the recovery of silver from photographic emulsions.

别 称

Proteinase from Bacillus licheniformis, Subtilisin A

含 量

7-15units/mg protein (biuret), (containing sodium benzoate and potassium sorbate)

CAS  NO.

9014-01-1

MDL  number

MFCD00132092

适用范围

生物试剂

l  理化信息

外 观

丙二醇水溶液

溶解性(25°C)

0.85% sodium chloride: soluble, clear (Diluted); H²O: miscible

l  生物学信息

生物活性/药理作用

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate.

应 用

Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations. Protease from Bacillus licheniformis has been used to hydrolyze muscle during the isolation of mitochondria from skeletal muscle. The enzyme has been used to digest the neurolemma in the antennal lobe of the moth; Heliothis virescens.

样品准备说明

This product gives a clear solution when diluted with 0.85%NaCl, and is completely miscible with water. It is recommended to prepare and use diluted solutions as needed, rather than storing at 2–8°C.

有效单位定义

One unit will hydrolyze casein to produce color equivalent to 1.0μmole (181μg) of tyrosine per min at pH7.5 at 37°C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov