Proteinase K from Tritirachium album
商品编号：
S0659
备注：
国外库存，货期2-4周，具体详情请联系销售人员。
详细介绍
l 基本信息 |
产品名称 | Proteinase K from Tritirachium album |
别 称 | Endopeptidase K |
含 量 | Protein, ≥90% biuret; ≥30units/mg protein | CAS
NO. | 39450-01-6 |
分子量 | mol wt 28.93 kDa; | MDL
number | MFCD00132129 |
适用范围 | 生物试剂, for molecular biology |
l 理化信息 |
外 观 | 白色至类白色冻干粉末 |
l 生物学信息 |
生物活性/药理作用 | Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA. |
应 用 | Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A. Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria Determination of enzyme localization on membranes Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling. Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. |
样品准备说明 | This product is soluble in water (1mg/ml), yielding a clear colorless solution. |
有效单位定义 | One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0μmole of tyrosine per min at pH7.5 at 37°C (color by Folin-Ciocalteu reagent). |
l 存储 |
存储温度 | -20°C |
l 注意事项及免责声明 |
本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。 |
l 参考文献 |
1. http://www.drugbank.ca 2. https://ncit.nci.nih.gov 3. https://www.ncbi.nlm.nih.gov |
