产品名称

Proteinase K from Tritirachium album

别 称

Endopeptidase K

含 量

≥30units/mg protein

CAS  NO.

39450-01-6

分子量

mol wt 28.93 kDa

MDL  number

MFCD00132129

适用范围

生物试剂

l  理化信息

外 观

白色至白色冻干粉末

溶解性(25°C)

H₂O: soluble 1 mg/mL, clear, colorless

l  生物学信息

生物活性/药理作用

Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA. Proteinase K has a broad specificity and degrades many proteins even in the native state. It mainly cleaves the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked α-amino groups. The optimum pH is between 7.5-9.0 and the isoelectric point is 8.9 Ca₂₊ (1-5mM) is required for activation. Proteinase K is inhibited by diisopropyl fluorophosphate (DFIP), and phenylmethanesulfonyl fluoride (PMSF).

应 用

The product is provided as a lyophilized powder. The product has been used to break down human lens protein. Protease footprinting by Proteinase K digestion can reveal protein-protein surface interactions. The enzyme from Sigma has been used in the pre-hybridization step of chicken embryos. It has also been used for the enrichment of PrPSc, a prion protein that is present in sheep, hamster and mouse scrapie samples. Proteinase K is useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. It is used for the removal of endotoxins bound to cationic proteins such as lysozyme and ribonuclease A.

It is useful for the isolation of hepatic, yeast, and mung bean mitochondria and is used to determine enzyme localization on membranes It is used for the treatment of paraffin embedded  tissue sections to expose antigen binding sites for antibody labeling and for digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. The product is provided as a lyophilized powder. The product has been used to break down human lens protein. Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.

Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria

Determination of enzyme localization on membranes

Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.

Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE)  research.

样品准备说明

This product is soluble in water (1mg/ml), yielding a clear colorless solution.

有效单位定义

One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0μmole of tyrosine per min at pH7.5 at 37°C (color by Folin-Ciocalteu reagent).

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov