产品名称

Enterokinase from bovine intestine

别 称

Enteropeptidase

含 量

recombinant, expressed in E. coli, ≥20units/mg protein, ≥95% (SDS-PAGE)

CAS  NO.

9014-74-8

MDL  number

MFCD00131020

适用范围

生物试剂

l  理化信息

物理形态

supplied as a solution in 20mM Tris-HCl, 200mM NaCl, and 50% glycerol

l  生物学信息

生物活性/药理作用

Enterokinase is a membrane bound serine protease that specifically and rapidly converts trypsinogen to trypsin, thereby, triggering the conversion of other zymogens to active enzymes. It has a molecular mass of approximately 150 kDa. The enzyme is a heterodimer, wherein, the light and the heavy chains are ed by two disulfide bridges. Native enterokinase is composed of an 800 amino acid heavy chain and a 235 amino acid light chain. It is a glycoprotein containing 35% carbohydrate. The polypeptide chain of trypsinogen is hydrolyzed only after an -(Asp)4-Lys- sequence. The enzyme is inhibited by soybean trypsin inhibitor. Enterokinase is typically used in protein modification and amino acid sequence determination.

应 用

Enterokinase is a member of the S1 peptidase family. In vivo, it is responsble for the proteolytic activation of trypsin from trypsinogen. Enterokinase is used for site specific cleavage of recombinant fusion proteins containing an accessible enterokinase recognition site for removal of affinity tags.

Enterokinase from bovine intestine has been used in a study to assess duodenase as a potential activator of cascade digestive proteases. Enterokinase from bovine intestine has also been used in a study to investigate an inhibitor of enteropeptidases and trypsin from the bovine duodenum.

The enzyme has been used to compare the specific activity with that of purified, recombinant bovine enterokinase (light chain) overexpressed in Escherichia coli.

样品准备说明

supplied as a solution in 20mM Tris-HCl, 200mM NaCl, and 50% glycerol

有效单位定义

One unit will produce 1.0 nanomole of trypsin from trypsinogen per min at pH5.6 at 25°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov