产品名称

Cathepsin B from human placenta

别 称

Cathepsin B1

含 量

≥5units/mg protein; Protein,~50% Lowry

CAS   NO.

9047-22-7

MDL   number

MFCD00130748

适用范围

生物试剂

l  理化信息

外 观

冻干粉

物理形态

Lyophilized powder containing phosphate buffer salts

溶解性(25°C)

H²O: soluble 1.0mg/mL, clear

l  生物学信息

生物活性/药理作用

Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Cathepsin B preferentially cleaves at the caboxyl side of Arg-Arg bonds in small molecule substrates. Cathepsin B has been shown to degrade β-amyloid precursor protein into harmless fragments . Cathepsin B inhibition has been shown to limit bone stasis in breast   cancer .

应 用

Cathepsin B is a lysosomal cysteine proteinase which hydrolyzes proteins with a broad specificity for peptide bonds. Cathepsin B may be a useful tool in Alzheimer’s research, as it may have a role in the natural defense against the disease .Cathepsin B may be used to cleave procaspase 1 and procaspase 11, and to induce apoptosis in digitonin-permeabilized cells.

样品准备说明

Dissolves in water at 1mg/mL concentration, yieliding a clear solution. Lyophilized powder containing phosphate buffer salts. This product is soluble in water (1mg/ml), yielding a clear solution.

有效单位定义

One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH6.0 at 40°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov