产品名称

Papain from papaya latex

别 称

Papainase

含 量

2×Crystallized, ≥16units/mg protein

CAS  NO.

9001-73-4

分子量

mol wt 21 kDa

MDL  number

MFCD00131791

适用范围

生物试剂

l  理化信息

外 观

白色至浅黄色至浅棕色缓冲水性悬浮液

物理形态

Suspension in 0.05M sodium acetate, pH4.5, containing 0.01% thymol.

l  生物学信息

生物活性/药理作用

A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.

pH optimum 6.0-7.0

Also hydrolyzes esters and amides.

Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.

. The pH optimum is 6.0-7.0

. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.

应 用

Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.

Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules.

样品准备说明

Suspension in 0.05M sodium acetate, pH4.5, containing 0.01% thymol.Immediately prior to use, the enzyme is typically diluted in buffer containing ~5mM L-cysteine.

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov