产品名称

Ficin from fig tree latex

一般描述

Extinction Coefficient: E_1% = 21.0 (280nm); pI: 9.0

含 量

≥1.0units/mg protein; Protein, 80-100%  biuret

CAS  NO.

9001-33-6

分子量

mol wt 23.8 kDa

MDL  number

MFCD00081597

适用范围

生物试剂

l  理化信息

外 观

白色至浅棕色冻干粉末

物理形态

Lyophilized powder containing L-cysteine and trace EDTA

l  生物学信息

生物活性/药理作用

Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported K^m for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F(ab′)₂ fragments from mouse IgG1.

样品准备说明

Lyophilized powder containing L-cysteine and trace EDTA;This enzyme is soluble in water (10mg/ml), yielding a clear solution.

有效单位定义

One unit will produce a ΔA²⁸⁰ of 1.0 per min at pH7.0 at 37°C when measuring TCA soluble products from casein in a final volume of 10ml (1cm light path).

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov