产品名称

Pepsin−Agarose from porcine gastric mucosa

一般描述

Pepsin is an enzyme found in gastric secretion.

别 称

Pepsin A

含 量

≥30units/mg dry solid

分子量

mol wt 35 kDa

MDL  number

MFCD00081840

适用范围

生物试剂

l  理化信息

外 观

白色至浅黄色至浅棕色冻干粉末

物理形态

Lyophilized powder stabilized with lactose

l  生物学信息

生物活性/药理作用

Preferentially cleaves C-terminal to Phe, Leu and Glu. It does not cleave at Val, Ala or Gly. pH optimum 2-4. Active in 4M urea and 3M guanidine HCl. Stable at 60°C. Pepsin is irreversibly inactivated at pH >6.

应 用

Pepsin immobilized onto agarose beads can be used to digest and then separate out the active peptides of the glycoprotein gliadin.

Pepsin-agarose has been used as an immobilization tool to investigate the refolding ability of the pepsin enzyme. It has also  been used for the preparation of F(ab′)² fragments from rabbit IgG.

Used to produce F(ab′)² fragments of antibodies.

样品准备说明

Lyophilized powder stabilized with lactose

有效单位定义

One unit will produce a ΔA²⁸⁰ of 0.001 per min at pH2.0 at 37°C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16mL. Light path = 1cm.)

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov