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Pepsin from porcine gastric mucosa

商品编号: S0695
图示 货号 库存 价格(¥) 数量
  • S0695-250MG

    有货

    616

    - +

    S0695-1G

    有货

    1171

    - +

    S0695-5G

    有货

    5031

    - +

    S0695-10G

    有货

    11401

    - +

    S0695-25G

    有货

    18850

    - +

备注:

国外库存,货期2-4周,具体详情请联系销售人员。

质量文档

详细介绍

l  基本信息

产品名称

Pepsin from porcine gastric mucosa

别 称

Pepsin A, Pepsin from hog stomach

含 量

≥2,500units/mg protein (E1%/280)

CAS  NO.

9001-75-6

分子量

mol wt 35 kDa

MDL  number

MFCD00081840

适用范围

生物试剂

l  理化信息

外 观

类白色至黄色至米色冻干粉末

溶解性(25°C)

deionized water: soluble 10mg/mL; 10mM HCl: soluble 4mg/mL (Cold)

l  生物学信息

生物活性/药理作用

Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin . Pepsin hydrolyzes peptide bonds, not amide or ester ages. Pepsin cleaves peptides with an aromatic acid on either side of the peptide bond. Sulfur-containing amino acids increase susceptibility to hydrolysis when they are close to the peptide bond. Pepsin preferentially cleaves at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid residues. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin

Pepsin is the major proteolytic enzyme produced in the stomach. It digests proteins through the cleavage of interior peptide ages.

The enzyme does not cleave at valine, alanine, or glycine ages. Z-L-tyrosyl-L-phenylalanine,  Z-L-glutamyl-L-tyrosine, or Z-L-methionyl-L-tyrosine may be used as substrates for pepsin digestion. Pepsin is inhibited by several phenylalanine-containing peptides.

应 用

Pepsin cleavage can be used to produce F(ab′)2 fragments of antibodies.

Pepsin is a peptidase used to digest proteins and is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice.

The enzyme has been used in the digestion of crude wheat gliadin. It has been used along with other enzymes to demonstrate the effects of fixation and enzymatic digestion in immunohistochemical assays, using paraffin embedded tissue. It has been used for digestion (before using immunoperoxidase techniques) to reduce non-specific background staining in sections of bronchial tissues. The enzyme  has also been used in the preparation of F(ab)2 fragment from IgG.

样品准备说明

Pepsin is soluble in deionized water at 1%(10mg/ml) and at 0.4%(4mg/ml) in cold 10mM hydrochloric acid.

有效单位定义

One unit will produce a ΔA280 of 0.001 per min at pH2.0 at 37°C, measured as TCA-soluble products using hemoglobin as substrate.(Final volume =16mL. Light path =1cm.)

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究,不得作为药物使用,不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov