产品名称

Thermolysin from Geobacillus stearothermophilus

一般描述

Thermolysin is a protease that has specificity different from other proteases available for sequence investigations.

别 称

Protease from Geobacillus stearothermophilus, Thermophilic-bacterial protease

含 量

Type X, 30-175units/mg protein (E1%/280)

CAS  NO.

9073-78-3

分子量

mol wt 34.6 kDa by amino acid sequence

MDL  number

MFCD00165549

适用范围

生物试剂

l  理化信息

外 观

白色至黄色至米色冻干粉末

l  生物学信息

应 用

A thermostable (thermophilic) extracellular lloendopeptidase containing four calcium ions. Cofactors are zinc and calcium. Hydrolyzes protein bonds on the N-terminal side of hydrophobic amino acid residues. The pH optimum is 8.0 and the optimal temperature for activity  is 70°C. Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others. Often used to do limited proteolysis for peptide mapping and studies of protein structure and conformational changes.

Thermolysin has been shown to have a prosequeence that acts as an intramolecular chaperone in vivo. It has also been used in a study to investigate the effects of sodium chloride on thermal stability and catalytic activity.

Thermolysin is also commonly used for the commercial synthesis of N-(benzyloxycarbonyl)-L-aspartyl-L-phenylalanine methyl ester, the precursor for the artificial sweetener aspartame.

样品准备说明

lyophilized powder containing calcium and sodium acetate buffer salts

有效单位定义

One unit will hydrolyze casein to produce color equivalent to 1.0μmole (181μg) of tyrosine per min at pH7.5 at 37°C (color by Folin-Ciocalteu reagent).

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov