产品名称

Thermolysin from Geobacillus stearothermophilus

别 称

Protease from Geobacillus stearothermophilus, Thermophilic-bacterial protease

含 量

30-175units/mg protein (E1%/280)

CAS  NO.

9073-78-3

MDL  number

MFCD00165549

适用范围

生物试剂, suitable for cell culture

l  理化信息

外 观

白色至黄色至米色粉末

物理形态

lyophilized powder containing calcium and sodium acetate buffer salts

l  生物学信息

应 用

A thermostable (thermophilic) extracellular lloendopeptidase containing four calcium ions. Cofactors are zinc and calcium. Hydrolyzes protein bonds on the N-terminal side of hydrophobic amino acid residues. The pH optimum is 8.0 and the optimal temperature for activity is 70°C. Considerably stable from pH5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others. Often used to do limited proteolysis for peptide mapping and studies of protein structure and conformational changes.

样品准备说明

lyophilized powder containing calcium and sodium acetate buffer salts.This product is soluble in acetate buffer, pH7.5 (0.2mg/ml), yielding a clear to very slightly hazy, colorless solution.      

有效单位定义

One unit will hydrolyze casein to produce color equivalent to 1.0μmole (181μg) of tyrosine per min at pH7.5 at 37°C (color by Folin-Ciocalteu reagent).

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov