产品名称

Collagenase from Clostridium histolyticum

别 称

Clostridiopeptidase A

含 量

Type VII, ≥4 FALGPA units/mg solid, 1,000-3,000 CDU/mg solid (CDU = collagen digestion units); Protein, ≥85% biuret

CAS  NO.

9001-12-1

MDL  number

MFCD00130830

适用范围

生物试剂

l  理化信息

物理形态

Lyophilized powder containing calcium chloride

溶解性(25°C)

TESCA buffer (50mM TES, 0.36mM Calcium chloride, pH7.4): soluble 0.05-0.1mg/mL at 37°C

l  生物学信息

生物活性/药理作用

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline. Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca₂₊) per mole enzyme. The culture  filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa.Optimal pH ranges from 6.3-8.8. The enzyme recognizes the sequence -R-Pro-8-X-Gly-Pro-R-, where X is most often a neutral amino acid.

应 用

Collagenase has been used in the preparation of arterial tissue for the study of advanced glycosylation end products (AGE). The enzyme has also been used along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, lung epithelium and many other tissues. It is also effective in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes.

样品准备说明

Lyophilized powder containing calcium chloride; A stock solution may be prepared by dissolving 0.05 - 0.1mg of collagenase in one mL of 50mM TES buffer containing 0.36mM calcium chloride (TESCA), pH7.4 at 37°C.

有效单位定义

One collagen digestion unit (CDU) liberates  peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH7.4 at 37°C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of  furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit  hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH7.6 at 25°C in the presence of DTT.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.  https://www.ncbi.nlm.nih.gov