产品名称

Collagenase + protease inhibitor

含 量

2-5 FALGPA units/mg solid, ≥800 CDU/mg solid

适用范围

生物试剂

l  理化信息

外 观

固体

l  生物学信息

生物活性/药理作用

Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four grams of calcium (Ca₂₊) per mole  of the enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase  treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5mg/mL are used for digestion. The duration of reaction can vary from 15 minutes to several hours for satisfactory cell dissociation without causing too much cell death. Zn₂₊ is required for activity. This product is used if the collagenase does not require a significant protease activity.

应 用

Collagenase with protease inhibitor has been used in a study to assess the association of immune system gene polymorphisms with quantitative features. It has also been used in a study to investigate the quantitative structure-activity relationship study on Clostridium histolyticum collagenase inhibitors. The enzyme from sigma has been used in the isolation of porcine pancreatic islets. It has also been used in the isolation of pancreatic-infiltrating lymphocytes from mice.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov