产品名称

Collagenase from Clostridium histolyticum

一般描述

Clostridium histolyticum is a pathogenic clostridium that produces collagenase. This is a mixture of enzymes containing collagenase, non-specific proteases and clostripain.

别 称

Clostridiopeptidase A

含 量

Type II, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid

CAS  NO.

9001-12-1

MDL  number

MFCD00130830

适用范围

生物试剂

l  理化信息

外 观

浅米色至米褐色和淡棕色至深棕色冻干粉末

l  生物学信息

生物活性/药理作用

Collagenase is activated by four gram atom calcium, per mole enzyme. It is inhibited by ethylene glycol-bis(β-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, β-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.Collagenase enzymes and neutral  protease plays an important role in the effective release of cells from  tissue.Collagenase recognizes the sequence -R-Pro-8-X-Gly-Pro-R-, where X is most often a neutral amino acid.

应 用

Collagenase may be used:

• for the preparation of arterial tissue for the study of advanced glycosylation end products (AGE)

• for use along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, lung epithelium and many other tissues.

• in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes

• for the preparation of viable hepatocytes  from rat liver and for the isolation of fat cells from rat adipose tissue

样品准备说明

Solutions are typically prepared at 1-2mg/mL in TESCA buffer (containing 50mM TES, 0.36mM Calcium chloride, pH7.4  at 37°C. This product also contains clostripain, nonspecific neutral protease, and tryptic activities.

注 意

As supplied, this product is stable for one year at -20°C. There is no loss in FALGPA or protease activity in 30 days at 37°C, 50°C and -20°C. Solutions of crude collagenase are stable if frozen quickly in aliquots (at 10mg/mL) and kept frozen at -20°C. Further freeze-thaw cycles will damage the solution. The product retains 100% activity over 7 hours when held on ice.

有效单位定义

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0μmole of leucine in 5 hours at pH7.4 at 37°C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit  hydrolyzes casein to produce color equivalent to 1.0μmole of tyrosine per 5 hr at pH7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0μmole of BAEE per min at pH7.6 at 25°C in the presence of DTT.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov