产品名称

Collagenase from Clostridium histolyticum

别 称

Clostridiopeptidase A

含 量

Type H, ≥1.0 FALGPA units/mg solid; ≥10units/mg solid

CAS  NO.

9001-12-1

MDL  number

MFCD00130830

适用范围

生物试剂

l  理化信息

外 观

浅米色至米褐色至深棕色粉末

l  生物学信息

生物活性/药理作用

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline. Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca₂₊) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5mg/mL is used for digestion. The duration of reaction varyies from 15 minutes to several hours to yield a satisfactory efficiency of cell dissociation, without causing too much cell death. Krebs Ringer buffer with calcium and BSA is preffered and Zn₂₊ is required for activity. This enzyme is tested for the release of hepatocytes at approximately 1mg/mL conc., in a total volume of 100mL for each rat liver.

应 用

Clostridiopeptidase A has been used in a study to investigate the synthesis and in vitro evaluation of macromolecular antitumour derivatives d on phenylenediamine mustard.Clostridiopeptidase  A has also been used in a study to investigate the strain-controlled enzymatic cleavage of collagen in loaded matrix. The enzyme has been used for the partial digestion of mouse abdominal muscle feed artery (AFA) during endothelial cell (EC) tube isolation and superfusion. This study investigated the electrical conduction along EC tubes from mouse feed arteries.The product has also been used during the preparation of bovine pulmonary artery endothelial cells. This bovine study investigated the contribution of nitric oxide to 20-hydroxyeicosatetraenoic acid-induced relaxation of pulmonary arteries.

有效单位定义

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH7.4 at 37°C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0μmole of tyrosine per 5 hr at pH7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0μmole of BAEE per min at pH7.6 at 25°C in the presence of DTT.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov