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Alpha-lytic protease

商品编号: S0738
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国外库存,货期2-4周,具体详情请联系销售人员。

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详细介绍

l  基本信息

产品名称

Alpha-lytic protease

一般描述

Alpha-lytic protease (aLP) is an alternative specificity protease for proteomics applications. This protease cleaves after T, A, S, and V residues. It generates peptides of similar average length as trypsin.

aLP was first isolated from the myxobacterium Lysobacter enzymogenes. The pro-form of aLP is 397 amino acids long. In its mature form, aLP is 198 amino acids long. Its tertiary structural core resembles those of pancreatic serine proteases.

Crystal structure studies of aLP have been reported. Several studies are available on the active site and catalytic mechanism of aLP. The role of the pro-region in the activation, secretion and folding of aLP has been studied.

The activity of aLP in the presence of various solution components is as follows:

• 0.1% sodium deoxycholate: ~1.75-fold enhanced activity

• 1.0% sodium deoxycholate: ~60% activity

• 0.1% SDS: ~50% activity

• 1.0% SDS: ~40% activity

• 1M guanidine HCl: ~20% activity

• 4M guanidine HCl: ~1% activity (essentially inactivated)

含 量

Alpha-lytic endopeptidase, alphaLP; ≥0.0005U/mg

适用范围

生物试剂

l  理化信息

物理形态

Supplied as a solution in 10mM sodium acetate buffer, pH5.0.

l  生物学信息

样品准备说明

Supplied as a solution in 10mM sodium acetate buffer, pH5.0.

有效单位定义

One unit will produce one mmole of p-nitroaniline per minute from N-succinyl-Ala-Ala-Ala-PNA at 25°C at pH7.5

l  存储

存储温度

−70°C

l  注意事项及免责声明

本产品仅用于实验研究,不得作为药物使用,不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov