产品名称

Furin human

含 量

≥2,000unit/mL, recombinant, expressed in baculovirus infected Sf9 cells

MDL  number

MFCD03456906

适用范围

生物试剂

l  理化信息

物理形态

Solution in 10mM MES, pH7.0 at 25°C,1mM CaCl², 50% glycerol.

l  生物学信息

生物活性/药理作用

Furin is a dibasic endoprotease that is localized in the Golgi apparatus. It is responsible for the proteolytic maturation of many precursor proteins in the secretory and endocytic pathways of mammalian cells.

Furin is a dibasic endoprotease that is localized in the Golgi apparatus. It has a molecular mass of 52.7 kDa. It is responsible for the proteolytic maturation of many precursor proteins in the secretory and endocytic pathways of mammalian cells. Furin cleaves precursor proteins at their paired basic amino acid processing sites. Some substrates of furin include von Willebrand factor, transforming growth factor beta 1 precursor, pro-beta-secretase and proparathyroid hormone.

应 用

Furin is capable of cleaving precursors of a wide variety of proteins, including growth factors, serum proteins, including proteases of the blood-clotting and complement systems, matrix lloproteinases, receptors, viral-envelope glycoproteins, and bacterial exotoxins, typically at sites marked by the consensus sequence Arg-Xaa-(Lys/Arg)-Arg.

样品准备说明

Solution in 10mM MES, pH7.0 at 25°C,1mM CaCl², 50% glycerol. Isolated from Spodoptera frugiperda (Sf9) cells infected with recombinant baculovirus carrying truncated human furin

有效单位定义

One unit is defined as the amount of Furin that will release 1pmole of AMC from the fluorogenic peptide Boc-RVRR-AMC in 1 minute at 30 °C.

l  存储

存储温度

−70°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov