产品名称

Penicillin Amidase from Escherichia coli

一般描述

Penicillin amidase is a periplasmic 80K heterodimer with A and B chains (209 and 566 amino acids, respectively). It  is widely distributed among microorganisms, including bacteria, yeast and filamentous fungi. Among all the sources, the enzyme produced by E. coli is most well-characterized and common for industrial application.

别 称

Penicillin Acylase, Penicillin  Amidohydrolase

含 量

5-10units/mg protein

CAS  NO.

9014-06-6

分子量

Mr ~70000

MDL  number

MFCD00147869

适用范围

生物试剂

l  生物学信息

生物活性/药理作用

The biosynthesis of Penicillin amidase in  E. coli by hydrophobic protein chromatography is an inducible reaction which is regulated by bolized carbon source (e.g. polyols, carboxylic acid etc.). It is also influenced by catabolite repression.It catalyzes the formation of amide bonds through an acyl-enzyme intermediate.

应 用

Penicillin amidase was used to study its effect in release of fatty acid and HSL (homoserine lactone) from AHLs (N  -acylhomoserine lactones) in degradation of antibiotics.It was used as positive control for assaying penicillin G acylase activity in the study of functional analysis of bile salt hydrolase and penicillin acylase family members in Lactobacillus sp.Penicillin amidase may be used for synthesis of 6-aminopenicillanic acid from penicillin-G and for the industrial production of β-lactam antibiotics.

有效单位定义

1U corresponds to the amount of enzyme which hydrolyzes 1μmol benzylpenicillin per minute at pH7.6 and 37°C

l  存储

存储温度

2-8°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov