产品名称

Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)

别 称

3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine amidohydrolase

含 量

≥100units/mg protein; Protein, ≥50% biuret

CAS  NO.

37289-07-9

MDL  number

MFCD00130789

适用范围

生物试剂

l  理化信息

外 观

白色至淡黄色至淡褐色冻干粉末

l  生物学信息

生物活性/药理作用

The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg₂₊,Cu₂₊, and Cd₂₊) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.

应 用

The enzyme has been used in the analysis of bile samples in various studies.

样品准备说明

Partially purified lyophilized powder containing buffer salts and stabilizer

有效单位定义

One unit will hydrolyze 1.0μmole of glycocholic acid to glycine and cholic acid in 5 min at pH5.6 at 37°C.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov