产品名称

PNGase F from Elizabethkingia  meningoseptica

别 称

N-Glycosidase F, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum, Peptide N-glycosidase

含 量

recombinant,expressed in E. coli, ≥1,000units/mg protein

CAS  NO.

83534-39-8

分子量

mol wt ~36 kDa

MDL  number

MFCD00131222

适用范围

生物试剂

l  理化信息

外 观

冻干粉

l  生物学信息

生物活性/药理作用

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

应 用

Highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-teminal 6x histidine fusion tag.Used to deglycosylate protein.

有效单位定义

One unit will catalyze the release of N-ed oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH7.5 monitored by SDS-PAGE.One unit of PNGase F activity is equal to 1 IUB milliunit.

l  存储

存储温度

-20°C

l  注意事项及免责声明

本产品仅用于实验研究，不得作为药物使用，不得用于家用或其它用途。

l  参考文献

1.    http://www.drugbank.ca

2.    https://ncit.nci.nih.gov

3.    https://www.ncbi.nlm.nih.gov